Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adeno-associated virus serotypes: vector toolkit for human gene therapy.

Zhijian Wu1, Aravind Asokan, R Jude Samulski

  • 1Gene Therapy Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|July 11, 2006
PubMed
Summary

Recombinant adeno-associated viral (AAV) vectors are crucial for gene therapy, offering diverse tissue targeting. This review explores novel AAV isolates, engineering strategies, and their implications for advanced gene delivery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural and evolved membrane-associated accessory proteins differentially engage SNARE machinery for AAV egress.

Journal of virology·2026
Same author

Functional characterization of furin-mediated lipoprotein lipase cleavage.

Disease models & mechanisms·2026
Same author

Erratum: Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors.

Molecular therapy. Advances·2026
Same author

Adeno-associated virus gene therapy-mediated CCR5 blockade suppresses virus replication long term in SHIV-infected macaques.

Science translational medicine·2026
Same author

Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

In vivo site-specific engineering to reprogram T cells.

Nature·2026

Area of Science:

  • * Gene Therapy
  • * Virology
  • * Molecular Biology

Background:

  • * Recombinant adeno-associated viral (AAV) vectors are key in modern gene therapy.
  • * Numerous naturally occurring AAV serotypes and variants enhance vector diversity and evade immunity.
  • * Understanding AAV tropism mechanisms is crucial for effective gene delivery.

Purpose of the Study:

  • * To review recent advancements in AAV vector isolation and characterization.
  • * To discuss AAV tissue tropisms, cellular entry, and trafficking mechanisms.
  • * To explore strategies for engineering novel AAV vectors for enhanced gene delivery.

Main Methods:

  • * Isolation and characterization of novel AAV serotypes and variants.
  • * Analysis of AAV capsid structure and surface topology.

Related Experiment Videos

  • * Engineering of hybrid AAV vectors using molecular cloning techniques.
  • * Investigation of cellular receptor usage and intracellular trafficking pathways.

    • Main Results:

    • * Diverse tissue tropisms observed in naturally occurring AAV isolates.
    • * Insights into mechanisms of selective AAV serotype tropism.
    • * Development of strategies for engineering tailored AAV vectors.
    • * Identification of AAV variants with potential for reduced immunogenicity.

    Conclusions:

    • * The expanding toolkit of AAV vectors offers significant potential for gene therapy.
    • * Further research into AAV biology and engineering is essential for clinical translation.
    • * Novel AAV isolates and engineered variants enhance specificity and efficacy in gene delivery.