[Microsatellite marker analysis of gynogenesis by artificial induction in Pseudosciaena crocea]

Gynogenesis was induced in two groups of Pseudosciaena crocea by the cool-shock and hydrostatic pressure method. The 24 fry in the gynogenetic families G1 and G2, 20 fry in their contrast groups and their parents were PCR amplified using 6 pairs of microsatellite marker primers LYC0002, LYC0003, LYC0013, LYC0012, LYC0004 and LYC0006 and analyzed. The results showed that the effect of cool-shock method, obtained a hatching rate of 35.3%, and a survival rate of 9.9% at 45 days, was obviously better than hydrostatic pressure method. Four pairs of primers could amplify clearly distinct parental bands. The results of primers LYC0012 and LYC0006 revealed no male gene in G1, suggesting they all derived from gynogenesis. Amplification using primers LYC0013 and LYC0004 in G2 revealed the presence of male genes in 3 individuals, suggesting they came from normal fertilization. The homozygosity ratio of the offspring from the two families was 87.5% and 76.2% and the average was 81.9%. They were 0 respectively in their contrast groups. The homozygous ratio of gene improved for 81.9% by gynogenesis. This study shows that gynogenesis is an effective method to promote gene purification In addition, microsatellite marker technique is an effective method of gynogenesis verification and genetic analysis.