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Related Experiment Videos

Sequence-based methods for identifying epidemiologically linked herpes simplex virus type 2 strains.

Emily Toth Martin1, David M Koelle, Benjamin Byrd

  • 1Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, 600 Broadway, Suite 400, Seattle, WA 98122, USA.

Journal of Clinical Microbiology
|July 11, 2006
PubMed
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Analyzing herpes simplex virus type 2 (HSV-2) DNA with DNA sequencing offers a more precise method for strain identification compared to traditional RFLP. This approach effectively differentiates related and unrelated viral infections.

Area of Science:

  • Virology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Traditional herpes simplex virus (HSV) identification relies on restriction fragment length polymorphism (RFLP), which has limitations in high-throughput analysis and distinguishing subtle strain variations.
  • The ability of RFLP to differentiate closely related HSV type 2 (HSV-2) strains, particularly in epidemiological contexts, remains unclear.

Purpose of the Study:

  • To compare the efficacy of RFLP and DNA sequencing for differentiating HSV-2 isolates from infected individuals and their sexual partners.
  • To evaluate the utility of sequence analysis across multiple genetic regions for precise HSV-2 strain identification.

Main Methods:

  • Analysis of HSV-2 DNA isolates from 14 primary infections and their sexual partners using RFLP and heteroduplex mobility assays.

Related Experiment Videos

  • Sequencing of seven HSV-2 regions, including noncoding and coding segments (gC, gB, gG genes), to identify sequence variations.

    • Main Results:

    • RFLP patterns were largely similar between partners, with minor differences in five couples, limiting its discriminatory power.
    • Heteroduplex mobility assays failed to distinguish between unrelated HSV-2 strains.
    • DNA sequencing identified 22 variations in 1,482 bp, differentiating all unrelated infections and confirming relatedness in all but two pairs.

    Conclusions:

    • Multilocus DNA sequencing is a more informative method than RFLP for distinguishing HSV-2 strains.
    • Sequence analysis holds significant potential for accurately identifying epidemiologically linked HSV-2 strains.