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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide
Kavita Imrit1, Michael Goldfischer, Jie Wang
1Department of Pathology, Clinical Microbiology Laboratory, Hackensack University Medical Center, 30 Prospect Ave., Hackensack, NJ 07601, USA.
Journal of Clinical Microbiology
|July 11, 2006
Abstract:
We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (
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