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Bacteroides dorei sp. nov., isolated from human faeces.
Mohammad Abdul Bakir1, Mitsuo Sakamoto1, Maki Kitahara1
1Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Summary
Researchers identified a novel bacterial species, Bacteroides dorei, from human feces. This discovery expands our understanding of the human gut microbiome and its associated bacterial diversity.
Area of Science:
- Microbiology
- Gut Microbiome Research
- Bacteriology
Background:
- Human feces harbor a diverse range of anaerobic bacteria.
- The genus Bacteroides is a significant component of the human gut microbiota.
- Accurate identification and classification of bacterial species are crucial for understanding host-microbe interactions.
Purpose of the Study:
- To characterize and classify two previously unknown bacterial isolates from human feces.
- To determine if these isolates represent a novel species within the Bacteroides genus.
Main Methods:
- Morphological and biochemical characterization of bacterial isolates.
- 16S rRNA gene sequencing for phylogenetic analysis.
- DNA-DNA hybridization to assess genomic relatedness.
Main Results:
- Two Gram-negative, anaerobic, rod-shaped bacteria were isolated.
- 16S rRNA gene sequencing confirmed placement within the Bacteroides genus and indicated novelty.
- Phenotypic, chemotaxonomic, and DNA-DNA homology analyses supported the designation of a single novel species.
Conclusions:
- The two isolates represent a single, novel species of the genus Bacteroides.
- The novel species is named Bacteroides dorei sp. nov.
- The type strain is JCM 13471(T) (=DSM 17855(T)).