[Investigation of calculating the FRET efficiency of donor-acceptor pair]

The research on the method of calculating FRET efficiency is important to organic white LED research. In the present article, a new method of calculating the FRET efficiency of donor-acceptor pair by means of emission and excitation spectra was investigated. This method is easy to use to process data, needs only normal experiment equipments and doesn't need the parameters such as fluorescence quantum yield. However it can only be used to calculate comparative result. With this method, the FRET efficiency of fluorescein sodium, alpha-naphthol, bromocresol purple and riboflavin with different concentrations was studied, and the results match the experimental results completely, so this method has proved to be reasonable.