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Published on: January 28, 2018
[Determination of nucleic acids using toluidine blue as a fluorescence probe]
Liang-qia Guo1, Fang-gui Ye, Xu-cong Lin
1Department of Chemistry, Fuzhou University, Fuzhou 350002, China.
Abstract:
A new fluorimetric method has been developed for the determination of deoxyribonucleic acid (DNA) with toluidine blue (TB) as a fluorescence probe. It is based on the fluorescence quenching of toluidine blue in the presence of DNA. In Tris-HCl buffer solution (pH 8.5), the calibration graph was linear over the range 0.10-6.00 microg x mL(-1) for ctDNA, and the detection limit was 27 ng x mL(-1). The result of the determination of DNA of camphor tree's leaf by this method was satisfactory.
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