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Related Experiment Videos

Improved human islet isolation using nicotinamide.

H Ichii1, X Wang, S Messinger

  • 1Diabetes Research Institute, Department of Epidemiology and Public Health, University of Miami Leonard M. Miller School of Medicine, Miami, Florida, USA.

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|July 11, 2006
PubMed
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Nicotinamide (NA) supplementation significantly improved islet yield and transplantation qualification rates during islet isolation. This enhancement was observed with both University of Wisconsin (UW) and two-layer (TLM) preservation methods.

Area of Science:

  • Transplantation immunology
  • Islet cell biology
  • Biomedical engineering

Background:

  • Islet isolation is crucial for type 1 diabetes treatment.
  • Current methods face challenges in maximizing islet yield and viability.
  • Nicotinamide (NA) is a potential agent to improve islet isolation outcomes.

Purpose of the Study:

  • To evaluate the effect of nicotinamide (NA) supplementation during islet isolation processing.
  • To compare NA's efficacy with two preservation methods: University of Wisconsin (UW) and two-layer method (TLM).

Main Methods:

  • Retrospective analysis of 102 human pancreata processed for transplantation.
  • Four groups were analyzed based on preservation (UW or TLM) and NA use (with or without NA).
  • Islet yield (IEQ/g) and qualification rates for transplantation were assessed.

Related Experiment Videos

Main Results:

  • Nicotinamide (NA) significantly increased islet yield in both UW (Group II vs. I) and TLM (Group IV vs. III) preservation methods.
  • Group IV (TLM with NA) showed higher islet yield than Group II (UW with NA).
  • NA supplementation increased the percentage of islet preparations qualifying for transplantation (25% to 47% for UW, 45% to 69% for TLM).

Conclusions:

  • Nicotinamide (NA) supplementation is effective in improving islet yield during isolation.
  • NA enhances islet isolation success regardless of the preservation method (UW or TLM).
  • The use of NA in processing significantly increases the proportion of islet preparations suitable for clinical transplantation.