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Updated: Aug 7, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
Published on: January 9, 2019
Urine flow cytometer quantification of leukocytes in samples containing a large proportion of lymphocytes
Hachiro Yamanishi1, Nobuko Imai, Toshinari Ohmine
1Laboratory for Clinical Investigation, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan. yamaha@hp-lab.med.osaka-u.ac.jp
Objective:
We compared leukocyte counts obtained by cytometric analysis and Fuchs-Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF).
Design And Methods:
UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation method was used.
Results:
The regression equation for UF and FR chamber counting of the cell suspensions was y=0.88x+18.8 WBC/microL (r=0.832, n=106). For a few high Lym% samples, markedly underestimated WBC counts were obtained by UF.
Conclusions:
Underestimated WBC count is due not to systematic error but to random error. Counts of the "other" population by UF may be useful for detection of underestimated samples.
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