Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PCAF modulates PTEN activity.

Koichi Okumura1, Michelle Mendoza, Robert M Bachoo

  • 1Ludwig Institute for Cancer Research, San Diego Branch, CA, USA.

The Journal of Biological Chemistry
|July 11, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Utrophin requires α-Syntrophin to maintain neuromuscular junction integrity in mdx mice.

Human molecular genetics·2026
Same author

Editorial Expression of Concern: YAP and MRTF-A, transcriptional co-activators of RhoA-mediated gene expression, are critical for glioblastoma tumorigenicity.

Oncogene·2026
Same author

Chromothripsis and ecDNA initiated by N4BP2 nuclease fragmentation of cytoplasm-exposed chromosomes.

Science (New York, N.Y.)·2025
Same author

Retraction of Activation of Rac1 by Src-dependent phosphorylation of Dock180Y1811 mediates PDGFRα-stimulated glioma tumorigenesis in mice and humans.

The Journal of clinical investigation·2025
Same author

BRD2 bromodomain-mediated regulation of cell state plasticity modulates therapy response in glioblastoma.

Neuro-oncology·2025
Same author

Reduction of neuronal activity mediated by blood-vessel regression in the adult brain.

Nature communications·2025

PTEN protein acetylation by PCAF regulates cell cycle and tumor suppression. This interaction, dependent on growth factors, links cancer pathways by controlling signaling and gene expression.

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Epigenetics

Background:

  • PTEN protein is crucial for suppressing tumor formation by regulating the cell cycle.
  • Mechanisms controlling PTEN activity in normal and cancerous cells are not fully understood.

Purpose of the Study:

  • To investigate the interaction between PTEN and PCAF (a histone acetyltransferase).
  • To elucidate the role of PCAF-mediated PTEN acetylation in regulating PTEN activity and cancer pathways.

Main Methods:

  • Studied the physical and functional interaction between PTEN and PCAF.
  • Analyzed PTEN acetylation at Lys125 and Lys128 residues using shRNA and PTEN mutants.
  • Assessed the impact on phosphatidylinositol 3-kinase/AKT signaling and cell cycle arrest.

Related Experiment Videos

Main Results:

  • PCAF expression increases PTEN acetylation at key residues (Lys125, Lys128) within its catalytic cleft.
  • This acetylation is growth factor-dependent and affects PTEN's ability to regulate PI3K signaling and cell cycle.
  • Acetylation-resistant PTEN mutants retain activity, confirming the causal role of acetylation.

Conclusions:

  • PCAF-mediated PTEN acetylation is a novel regulatory mechanism for PTEN.
  • This process links growth factor signaling pathways with gene expression control in cancer.
  • Findings reveal a new therapeutic target for cancer treatment.