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Related Experiment Videos

Candida-induced oral epithelial cell responses.

E A Lilly1, J E Leigh, S H Joseph

  • 1Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center and School of Dentistry, New Orleans, LA, USA.

Mycopathologia
|July 11, 2006
PubMed
Summary

Oral epithelial cells from HIV(+) individuals produce low levels of cytokines in response to Candida albicans. Cytokines do not appear to enhance the anti-Candida activity of these cells.

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Area of Science:

  • Immunology
  • Oral Microbiology
  • Infectious Diseases

Background:

  • Oropharyngeal candidiasis (OPC) is a common oral infection in HIV(+) individuals.
  • Oral epithelial cells play a role in innate immunity against OPC by producing cytokines and inhibiting Candida growth.
  • Understanding the interaction between oral epithelial cells, Candida albicans, and cytokines in HIV(+) persons is crucial for managing OPC.

Purpose of the Study:

  • To investigate if oral epithelial cells from HIV(+) persons, with or without OPC, produce cytokines when exposed to Candida albicans.
  • To determine if cytokines influence the anti-Candida activity of oral epithelial cells.

Main Methods:

  • Oral epithelial cells from HIV(+) individuals (with and without OPC) were cultured with Candida albicans.

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  • Supernatants were analyzed for cytokine levels using ELISA.
  • Cytokines were added to growth inhibition assays using oral epithelial cells from HIV(-) individuals.

    • Main Results:

    • Oral epithelial cells from HIV(+) persons showed low cytokine production in response to Candida.
    • Individuals with OPC had elevated levels of TNF-alpha and IL-6 compared to those without OPC.
    • Added cytokines did not affect the anti-Candida activity of oral epithelial cells from HIV(-) persons.

    Conclusions:

    • Oral epithelial cells from HIV(+) persons contribute to the oral cytokine environment during OPC.
    • Cytokines do not appear to modulate the intrinsic anti-Candida activity of oral epithelial cells.