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Updated: Aug 7, 2026

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
Published on: May 10, 2014
Electrolyte redistribution in cystic fibrosis fibroblasts studied by electron probe X-ray microanalysis
G M Roomans1, O Ceder, H Kollberg
1Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S-11345 Stockholm, Sweden.
Abstract:
The elemental composition of fibroblasts from cystic fibrosis (CF) patients and from healthy controls was compared by means of electron probe X-ray micro-analysis. Significantly lower sodium levels (p < .01) and higher calcium levels (p < .02) were found in CF fibroblasts, indicating a disturbance of ion regulation in these cells. Treatment with Staphylococcus aureus protein A and gammaglobulin (proteins that may influence the ciliotoxic CF factor) did not change the elemental composition of the fibroblasts.
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