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12:24
Murine Model of CD40-activation of B cells
Published on: March 6, 2010
The bovine p150/95 molecule (CD11c/CD18) functions in primary cell-cell interaction
L Eskra1, K L O'Reilly, G A Splitter
1Department of Veterinary Science, University of Wisconsin-Madison 53706.
Veterinary Immunology and Immunopathology
|September 1, 1991
Summary
The monoclonal antibody C5B6 targets the CD11c/CD18 molecule on bovine monocytes, inhibiting primary immune responses to alloantigens. This antibody shows specificity for certain bovine lymphocytes but not other species.
Area of Science:
- Immunology
- Veterinary Science
- Cell Biology
Background:
- Monoclonal antibodies (MAbs) are crucial tools for identifying cell surface molecules.
- The CD11c/CD18 molecule is a key component of the leukocyte adhesion receptor family.
- Understanding bovine immune cell markers aids in diagnosing and treating animal diseases.
Purpose of the Study:
- To characterize the specificity and function of the monoclonal antibody C5B6.
- To investigate the role of the CD11c/CD18 molecule in bovine immune cells.
- To evaluate the potential of C5B6 as an immunomodulatory agent in cattle.
Main Methods:
- Flow cytometry was used to determine MAb C5B6 reactivity with bovine peripheral blood leukocytes.
- Immunoprecipitation assays were performed to identify the molecular weight of antigen recognized by C5B6.
- Functional assays assessed the effect of C5B6 on lymphocyte proliferation and cytotoxicity.
Main Results:
- MAb C5B6 specifically recognized the CD11c/CD18 molecule on bovine monocytes and granulocytes.
- Immunoprecipitation revealed bands of 150,000 and 95,000 Da, consistent with the CD11c/CD18 complex.
- C5B6 inhibited the primary but not secondary proliferative response to alloantigens, without affecting cytotoxicity.
Conclusions:
- MAb C5B6 is a valuable reagent for identifying bovine monocytes and granulocytes expressing CD11c/CD18.
- The CD11c/CD18 molecule plays a role in the primary immune response to alloantigens in cattle.
- C5B6 demonstrates potential for modulating specific immune responses in bovine applications.
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