[Effects of nitric oxide synthases on kidney in early phase of endotoxic shock in macaque]
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Updated: Aug 7, 2026
5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
Published on: July 3, 2013
Ming Ma1, Xiao-yun Zhu, Li-rong Wang
1Department of Medicine, The Second Hospital of Nanjing, Medical College, South East University, Nanjing 210003, Jiangsu, China. maming_1971@yahoo.com.cn
Objective:
To study the pathogenesis of kidney injury in early phase of endotoxic shock.
Methods:
Eleven macaques were anesthetized and randomly divided into two groups: an experimental group (n=6), receiving a dose of 2.8 mg/kg lipopolysaccharides (LPS) by intravenous injection, and a control group (n=5), injected with normal saline of 1 ml/kg. The animals were sacrificed 120 minutes following endotoxin injection. With immunohistochemical technique endothelium nitric oxide synthases (eNOS) and inducible nitric oxide synthases (iNOS) protein of the renal tissue were determined. The ultrastructure was studied with electron microscope. Acid phosphatase (ACP) was also assayed.
Results:
One hundred and twenty minutes after LPS challenge, damages to the glomerulus and renal tubules were found, and lysosomes were increased in the renal cells. iNOS was positively expressed in vascular endothelium of glomerulus and epithelium of renal tubules in the experimental group, but not in the control group. eNOS was positively expressed in vascular endothelium of glomerulus; and peri-vascular region in both groups.
Conclusion:
The present findings indicate that NO contributed to the pathogenesis of renal injury in the early phase of endotoxic shock in primates.
