Polypeptides synthesized in rabbit cells infected with murine herpesvirus (MHV): a comparison of proteins specified by various MHV strains
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1Department of Microbiology and Virology, Faculty of Natural Sciences of Comenius University, Bratislava, Czecho-Slovakia.
Abstract:
Polypeptides synthesized in rabbit embryo fibroblasts (REF) infected with six isolates of murine herpesvirus (MHV) coming from different localities in CSFR were compared by electrophoresis (SDS-PAGE). Altogether 28 virus-coded polypeptides with apparent molecular weights ranging from 240 kD to 16 kD were identified. While isolates MHV-60, MHV-72, and MHV-76 had identical polypeptide profiles, the isolates MHV-68 and MHV-78 lacked a polypeptide with apparent molecular weight of 46 kD, the rest of polypeptides being identical as well. The polypeptide profile of MHV Sumava A.f. was most different from that of other MHV isolates. This result is in agreement with some different biological properties of this isolate. At a high multiplicity of infection (40 TCID50 per cell) first polypeptides with apparent molecular weights 125 kD and 84 kD were detected by 5-9 hr post-infection (p.i.). All other proteins appeared 14-18 p.i.
Insights
Murine herpesvirus (MHV) isolates from different regions were compared using SDS-PAGE. Variations in polypeptide profiles were observed, with one isolate showing distinct differences, correlating with its unique biological properties.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Murine herpesvirus (MHV) is a significant pathogen affecting various animal models.
- Understanding MHV isolate diversity is crucial for disease control and research.
- Previous studies have indicated potential variations among MHV strains.
Purpose of the Study:
- To compare the polypeptide profiles of six different murine herpesvirus (MHV) isolates.
- To identify variations in virus-coded proteins that may correlate with biological differences.
- To characterize the temporal expression of MHV-induced polypeptides.
Main Methods:
- Rabbit embryo fibroblasts (REF) were infected with six MHV isolates.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze synthesized polypeptides.
- Virus-coded polypeptides were identified based on their apparent molecular weights.
Main Results:
- A total of 28 virus-coded polypeptides, ranging from 240 kD to 16 kD, were identified across the MHV isolates.
- Three isolates (MHV-60, MHV-72, MHV-76) exhibited identical polypeptide profiles.
- Isolates MHV-68 and MHV-78 lacked a 46 kD polypeptide, while MHV Sumava A.f. displayed the most distinct profile, aligning with its unique biological characteristics.
- Early polypeptide expression (125 kD and 84 kD) was detected 5-9 hours post-infection, with other proteins appearing 14-18 hours post-infection.
Conclusions:
- SDS-PAGE analysis revealed distinct polypeptide profiles among MHV isolates, indicating genetic or regulatory variations.
- The unique polypeptide profile of the MHV Sumava A.f. isolate supports observations of differing biological properties.
- Temporal analysis of polypeptide synthesis provides insights into the MHV replication cycle.