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Gamma irradiation alters bluetongue virus protein antigen
A Afshar1, P Moreau, G C Dulac
1Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Acta Virologica
|May 1, 1991
Abstract:
Bluetongue virus (BTV) antigen, prepared for a monoclonal antibody (MAb)-based competitive enzyme-linked immunosorbent assay (C-ELISA), was exposed to 1, 2, 3, 4, 5 and 6 Mrad of gamma irradiation. The major group-specific BTV protein (VP7) reactive with the Mab was altered at higher doses of radiation, as revealed by immunoblotting studies. As well, a reduction in immunoreactivity was noted when irradiated antigen was used in the ELISA.