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Updated: Aug 7, 2026
Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
Published on: March 1, 2019
[Development of radioimmunoassay for human sperm protein 17]
Guo-hong Wang1, Fang-qiu Li, Wei Sun
1Center of Medical Laboratory Sciences of PLA, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Objective:
To develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.
Methods:
Anti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.
Results:
The assay range was 3.3 to approximately 800 microg/L, the sensitivity was 2.0 microg/L, and the intra- and inter-assay coefficients of variation (CV) were 7.5% to approximately 9.8% and 8.2% to approximately 13.2%, respectively. The serum Sp17 level in normal subjects was (15.60 +/- 7.66) microg/L (n = 59).
Conclusion:
This radioimmunoassay of Sp17 fulfills the reasonable requirements of clinical routine and scientific studies in terms of specificity, sensitivity and practicability. Measurement of Sp17 concentration is useful for assessing its native distribution and aberrant expression.
