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Updated: Jul 10, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Metabolic coupling of dehydration and decarboxylation in the curacin A pathway: functional identification of a
Liangcai Gu1, Junyong Jia, Haichuan Liu
1Life Sciences Institute, Department of Medicinal Chemistry, Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
Abstract:
This study describes the functional identification of a pair of mechanistically diverse enzymes that catalyze the successive dehydration (CurE ECH1) and decarboxylation (CurF ECH2) of (S)-HMG-ACP to generate a 3-methylcrotonyl-ACP intermediate, the presumed precursor of the cyclopropyl ring in curacin A. The reactions catalyzed by ECH1 and ECH2 are found in a broad cross-section of microbial natural product gene clusters and participate in the introduction of carbon chain branch points and functional group diversity as key steps in the HMG-CoA synthase mediated addition of C-2 from acetate to the beta-carbonyl group of polyketide chains.
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