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Related Experiment Videos

Lactic acid bacteria isolated from canine faeces.

S S Beasley1, T J K Manninen, P E J Saris

  • 1Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland.

Journal of Applied Microbiology
|July 13, 2006
PubMed
Summary

Researchers identified specific lactic acid bacteria (LAB) from dog feces. Five strains show probiotic potential for canines due to acid tolerance and antimicrobial activity.

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Area of Science:

  • Microbiology
  • Animal Science
  • Probiotics

Background:

  • Lactic acid bacteria (LAB) are crucial for gut health.
  • Identifying host-specific LAB can lead to targeted probiotic development.
  • Canine gut microbiota research is essential for animal well-being.

Purpose of the Study:

  • To isolate and characterize lactic acid bacteria (LAB) from healthy dog feces.
  • To evaluate the probiotic potential of selected LAB strains for canine use.
  • To identify host-specific LAB with antimicrobial properties.

Main Methods:

  • Isolation and 16S rRNA gene sequencing of LAB from canine fecal samples.
  • Selection of candidate probiotic strains based on frequency, growth, acid tolerance, and antimicrobial activity.

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  • Determination of minimal inhibitory concentrations and viability at low pH.

    • Main Results:

    • LAB were detected in 67% of canine fecal samples.
    • Five LAB strains (Lactobacillus fermentum LAB8, L. mucosae LAB12, L. rhamnosus LAB11, L. salivarius LAB9, and Weissella confusa LAB10) were selected.
    • Selected strains demonstrated acid tolerance (viable at pH 2 for 4h) and antimicrobial activity against Micrococcus luteus.

    Conclusions:

    • Thirteen LAB species were identified in healthy canine feces.
    • Five acid-tolerant, antimicrobial LAB strains were chosen as potential canine probiotics.
    • These host-specific LAB represent a novel source for canine probiotic development.