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Isolation from poultry litter and characterization [corrected] of Staphylococcus spp. capable of growth in high
Y Vadari1, B P Mason, K C Doerner
1Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA.
Aims:
To isolate and characterize micro-organisms from poultry litter capable of growing under phosphate concentrations typical of poultry litter.
Methods And Results:
Poultry litter extracts were plated onto brain-heart infusion medium (BHI) containing an additional 0.75 mol l(-1) phosphate (BHI-P). Colonies were screened for the presence of inclusion granules with five being selected for further study. All strains displayed identical biochemical characteristics consistent with Staphylococcus spp. and grouped with Staphylococcus spp. by comparative 16S rDNA analysis. Thus all five strains were identified as such. All strains displayed elevated intracellular phosphate levels when cultured in BHI-P broth (0.417-0.600 microg phosphate mg(-1) protein) vs BHI broth (0.075-0.093 microg phosphate mg(-1) protein). When grown using an austere semi-defined medium or BHI-P, Staph. sp. #7 displayed similar elevated intracellular phosphate levels compared with growth in BHI.
Conclusions:
Poultry litter contains novel Staphylococcus spp. capable of robust growth when exposed to phosphate levels comparable with that typically found in poultry litter. Data suggest intracellular phosphate levels in these strains increase in response to increasing phosphate in the medium or austere medium conditions. Intracellular phosphate did not reach levels comparable with known hyper-accumulating micro-organisms.
Significance And Impact Of The Study:
These data suggest poultry litter possesses a resident microflora that thrives and accumulates intracellular phosphate in response to high phosphate conditions.
Insights
Poultry litter harbors novel Staphylococcus species that thrive in high phosphate conditions. These microbes accumulate intracellular phosphate, indicating a unique adaptation to their environment.
Area of Science:
- Microbiology
- Environmental Science
- Biochemistry
Background:
- Poultry litter is rich in phosphate due to animal waste.
- Microbial adaptation to high-phosphate environments is crucial for nutrient cycling.
Purpose of the Study:
- To isolate and characterize microorganisms from poultry litter that can grow under high phosphate concentrations.
- To investigate the phosphate accumulation capabilities of these isolated microorganisms.
Main Methods:
- Isolation of microorganisms from poultry litter using brain-heart infusion medium with added phosphate (BHI-P).
- Biochemical characterization and 16S rDNA analysis for species identification.
- Quantification of intracellular phosphate levels under varying phosphate conditions.
Main Results:
- Five strains of Staphylococcus spp. were isolated and identified.
- All identified strains demonstrated robust growth in high-phosphate medium (BHI-P).
- Elevated intracellular phosphate levels were observed in Staphylococcus spp. when cultured in BHI-P compared to standard BHI.
Conclusions:
- Poultry litter contains Staphylococcus species adapted to high phosphate levels.
- These microbes accumulate intracellular phosphate in response to their environment.
- While phosphate accumulation occurs, it does not reach levels seen in known hyper-accumulating organisms.
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