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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
Published on: August 25, 2018
Expression profiling of human epidermal keratinocyte response following 1-minute JP-8 exposure
Chi-Chung Chou1, Jen-Hung Yang, San-Duo Chen
1Department of Veterinary Medicine and Veterinary Medicine Teaching Hospital, National Chung-Hsing University, Taichung, Taiwan, ROC. ccchou@nchu.edu.tw
Abstract:
The cDNA microarray analysis of 9600 expressed sequence tags was performed to examine the gene expression changes in human epidermal keratinocytes after 1-minute JP-8 exposure; 151 genes were identified as JP-8 responsive and classified into 8 clusters by self organization map. Genes involved in basal transcription and translations were up-regulated, whereas genes related to DNA repair, metabolism, and keratin were mostly down-regulated. Genes encoded for growth factors, apoptosis, signal transduction, and adhesion were also altered. These results indicated that human keratinocyte responds to a single dose of JP-8 insult and revealed several cellular processes previously not associated with jet fuel exposure.
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