Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A role for P2X7 in microglial proliferation.

Fabio Bianco1, Stefania Ceruti, Alessio Colombo

  • 1CNR-Institute of Neuroscience, Milan, Italy.

Journal of Neurochemistry
|July 14, 2006
PubMed
Summary

Lipopolysaccharide (LPS) reduces microglial proliferation by decreasing P2X7 receptor expression. Blocking this purinoreceptor or its signaling pathway inhibits microglial cell cycle progression, highlighting P2X7

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Proteomic Analysis of Cerebrospinal Fluid From Neurological Patients With and Without Amyotrophic Lateral Sclerosis.

Journal of neurochemistry·2026
Same author

Rethinking hypercortisolism screening in obesity and diabetes: A systematic review and meta-analysis.

Metabolism: clinical and experimental·2026
Same author

Skin Application of Inflammasome Inhibitor MCC950 Prevents Psoriasis-like Cutaneous Manifestations and Associated Systemic Inflammation.

Inflammation·2026
Same author

Dyslipidemias associated with endocrine disorders: a position statement of the working group of the nutrition hormones and metabolism club of the italian society of endocrinology (SIE).

Journal of endocrinological investigation·2026
Same author

Sex differences in classic congenital adrenal hyperplasia: a multicenter, real-world analysis.

Frontiers in endocrinology·2026
Same author

Usability Comparison Between Leap Motion Controller and MediaPipe in Serious Games.

Studies in health technology and informatics·2026

Area of Science:

  • Neuroimmunology
  • Cell Biology

Background:

  • Microglia are CNS immune cells that alter their phenotype in response to stimuli.
  • Lipopolysaccharide (LPS) activates microglia, mimicking bacterial infection and causing changes in morphology, proliferation, and purinoreceptor signaling.
  • Activated microglia exhibit decreased P2X7 receptor expression.

Purpose of the Study:

  • To investigate the role of P2X7 receptor down-regulation in LPS-induced microglial antiproliferative effects.
  • To determine if P2X7 receptor signaling is crucial for microglial proliferation.

Main Methods:

  • Utilized P2X7 receptor antagonists (oxidized ATP, KN62, Brilliant Blue G) and ATP-hydrolase apyrase to block P2X7 receptor function.
  • Employed small interfering RNA (siRNA) to down-regulate P2X7 receptor expression in primary microglia and N9 clones.

Related Experiment Videos

  • Performed flow cytometry to analyze cell cycle progression and apoptosis in response to LPS and P2X7 receptor modulation.

    • Main Results:

    • Chronic blockade of the P2X7 receptor significantly reduced microglial proliferation.
    • siRNA-mediated down-regulation of P2X7 receptor expression also decreased microglial proliferation.
    • P2X7 receptor-deficient microglia (N9 clones and primary cells) showed unaffected proliferation with LPS or P2X7 antagonists.
    • LPS and oxidized ATP treatment reversibly decreased cell cycle progression without inducing apoptosis.

    Conclusions:

    • The P2X7 receptor plays a critical role in controlling microglial proliferation.
    • P2X7 receptor signaling supports microglial cell cycle progression.
    • Down-regulation of the P2X7 receptor is a key mechanism underlying the antiproliferative effect of LPS on microglia.