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Updated: Aug 7, 2026
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
[Prevalence of putative periodontal microorganisms in Chinese patients with aggressive periodontitis]
Xiang-hui Feng1, Li Zhang, Huan-xin Meng
1Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Objective:
To evaluate the prevalence of putative periodontal microorganism in Chinese patients with aggressive periodontitis (AgP).
Methods:
A total of 72 subgingival plaque samples were collected from 55 AgP patients and 17 healthy subjects. Seven putative periodontal microorganisms including Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf), Treponema denticola (Td), Campylobacter rectus (Cr), Prevotella intermedia (Pi) and Prevotella nigrescens (Pn) were detected by using 16S rRNA based polymerase chain reaction (PCR).
Results:
The prevalence of Aa in AgP patients was very low (1.8%), while Pg, Tf, Td and Cr were more frequently detected in AgP patients (prevalence: 81.8%, 83.6%, 80% and 81.8%) than in healthy controls (prevalence: 17.6%, 11.8%, 5.9%, 29.4%, P < 0.01).
Conclusions:
The prevalence of Pg, Tf, Td and Cr were very high in aggressive periodontitis patients, and the combination of these bacteria may play an important role in AgP.
