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Related Experiment Videos

Size-exclusion chromatography using deuterated mobile phases.

Jeanine M Erdner1, Howard G Barth, Joe P Foley

  • 1Corporate Center for Analytical Sciences, DuPont Company, Wilmington, DE 19880-0228, USA.

Journal of Chromatography. A
|July 14, 2006
PubMed
Summary

Deuterated solvents in size-exclusion chromatography (SEC) minimally impact elution volume but can decrease column efficiency. Adsorption was not observed, but temperature affects polymer hydrodynamic volume.

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Area of Science:

  • Polymer Science
  • Analytical Chemistry
  • Chromatography

Background:

  • Size-exclusion chromatography (SEC) is a crucial technique for polymer characterization.
  • The choice of solvent can influence chromatographic performance and polymer behavior.
  • Deuterated solvents are sometimes used to avoid interference in specific detection methods.

Purpose of the Study:

  • To investigate the effects of deuterated solvents on SEC parameters.
  • To assess polymer adsorption and column efficiency in the presence of deuterated solvents.
  • To compare the behavior of water-soluble polymers in H2O and D2O.

Main Methods:

  • Intrinsic viscosity measurements
  • SEC calibration curves
  • Column efficiency analysis

Related Experiment Videos

  • Exclusion experiments at varying temperatures (35°C and 50°C)

    • Main Results:

    • Deuterium oxide (D2O) slightly increased SEC elution volume for aqueous systems.
    • No significant polymer adsorption was detected for pullulan or polyethylene glycol/poly(ethylene oxide) (PEG/PEO).
    • PEG/PEO showed hydrodynamic volume contraction at higher temperatures in both H2O and D2O.
    • D2O caused moderate band broadening, indicating a decrease in column efficiency.
    • Chloroform vs. deuterated chloroform showed no hydrodynamic volume changes.

    Conclusions:

    • Deuterated solvents have a minor impact on SEC elution volumes but can affect column efficiency.
    • Temperature plays a role in the hydrodynamic volume of certain polymers.
    • The use of D2O in SEC is viable, with careful consideration of potential band broadening.