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Updated: Aug 7, 2026
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CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
Published on: December 8, 2023
Discovery of urinary biomarkers
Trairak Pisitkun1, Rose Johnstone, Mark A Knepper
1Laboratory of Kidney and Electrolyte Metabolism, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA.
Molecular & Cellular Proteomics : MCP
|July 14, 2006
Summary
Urinary protein and peptide profiling using mass spectrometry can aid disease diagnosis and monitoring. Advanced LC-MS/MS methods offer hope for faster discovery and validation of crucial urinary biomarkers.
Area of Science:
- Proteomics
- Biomarker Discovery
- Clinical Chemistry
Background:
- Human urine contains diverse proteins and peptides from various physiological processes.
- Urinary proteomic profiling holds potential for early disease diagnosis, prognosis, and therapy monitoring.
- Kidney and urinary tract diseases, as well as systemic conditions, may exhibit detectable urinary biomarkers.
Purpose of the Study:
- To review the application of mass spectrometry-based urinary protein and peptide profiling for biomarker discovery.
- To focus on progress in bladder cancer and acute renal transplant rejection.
- To highlight the potential of advanced mass spectrometry techniques for biomarker validation.
Main Methods:
- Review of mass spectrometry-based proteomic approaches, including two-dimensional electrophoresis and top-down mass spectrometry (SELDI-TOF-MS, CE-MS).
- Focus on applications in nephrology, urology, and oncology.
- Discussion of emerging liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods.
Main Results:
- Previous large-scale studies primarily utilized 2D electrophoresis or top-down MS.
- Applications have been mainly in nephrology, urology, and oncology, with limited progress to date.
- Emerging LC-MS/MS techniques show promise for accelerated biomarker discovery.
Conclusions:
- Urinary proteomic profiling is a promising field for identifying disease biomarkers.
- Despite past limitations, advancements in LC-MS/MS offer significant potential for future biomarker discovery and validation.
- This approach could revolutionize the diagnosis and management of various diseases.
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