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Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CpG DNA stimulation
S Olishevsky1, A Burlaka, E Sidorik
1R.E. KavetskyInstitute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine. sergeyolishevsky@yahoo.com
Aim:
To investigate the features of metabolic activation induced by bacterial CpG DNA (bCpG DNA) in peritoneal macrophages (PMphis).
Methods:
Electron paramagnetic resonance spin-trapping technique using respective spin traps was applied to study the generation rate of reactive oxygen species and NO production by PMphis of BALB/c mice.
Results:
For the first time the capability of bCpG DNA isolated from Bacillus subtilis GP1-807-03 culture medium to elevate activity of NADP composite functionH oxidase and inducible NO synthase in PMphis of normal and tumor-bearing mice have been demonstrated. The main differences in superoxide anion generation rate and production of NO by PMphis of normal mice and mice with transplanted solid Ehrlich carcinoma were showed. The effects of bCpG DNA stimulation in vitro on ROS and NO production by PMphis depended on concentration and time exposure with bCpG DNA. Furthermore, response of PMphis from tumor-bearing mice on bCpG DNA stimulation was delayed as compared to PMphis of normal mice.
Conclusion:
The present findings suggest that bCpG DNA have modulatory effect on ROS/NO production by PMphis from normal and tumor-bearing animals.
Insights
Bacterial CpG DNA (bCpG DNA) activates metabolic processes in mouse peritoneal macrophages (PMphis), influencing reactive oxygen species (ROS) and nitric oxide (NO) production. This immune response differs between normal and tumor-bearing mice.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Bacterial CpG DNA (bCpG DNA) is known to activate immune cells.
- Peritoneal macrophages (PMphis) play a crucial role in innate immunity.
- Understanding immune activation in tumor-bearing states is vital for therapeutic strategies.
Purpose of the Study:
- To investigate the metabolic activation of peritoneal macrophages (PMphis) induced by bacterial CpG DNA (bCpG DNA).
- To analyze the production of reactive oxygen species (ROS) and nitric oxide (NO) by PMphis upon bCpG DNA stimulation.
- To compare the responses of PMphis from normal and tumor-bearing mice.
Main Methods:
- Utilized electron paramagnetic resonance (EPR) spin-trapping techniques.
- Quantified reactive oxygen species (ROS) and nitric oxide (NO) generation rates.
- Studied PMphis isolated from normal BALB/c mice and those with transplanted solid Ehrlich carcinoma.
Main Results:
- Demonstrated that bCpG DNA from Bacillus subtilis enhances NADP(H) oxidase and inducible NO synthase activity in PMphis.
- Observed distinct differences in ROS and NO production rates between normal and tumor-bearing mice.
- Found that bCpG DNA stimulation effects on ROS/NO production were dose- and time-dependent.
- Noted a delayed response to bCpG DNA in PMphis from tumor-bearing mice compared to normal mice.
Conclusions:
- Bacterial CpG DNA (bCpG DNA) exhibits a modulatory effect on ROS and NO production in peritoneal macrophages (PMphis).
- These findings highlight differential immune activation patterns in normal versus tumor-bearing states, influenced by bCpG DNA.
