Related Experiment Video
Updated: Jan 1, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus
Sonoko Ishino1, Yoshizumi Ishino
1Department of Molecular Microbiology, Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Abstract:
DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containingDNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.
Related Concept Videos
Diversity of Archaea IV
Diversity of Archaea III
Diversity of Archaea I
Hyperthermophilic Bacteria
PCR
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

