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Updated: Aug 7, 2026
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
Published on: October 30, 2011
Human neuroblastoma stem cells
Robert A Ross1, Barbara A Spengler
1Laboratory of Neurobiology, Department of Biological Sciences, 441 East Fordham Road, Fordham University, Bronx, NY 10458, USA. rross@fordham.edu
Abstract:
Human neuroblastoma is an embryonic cancer of the neural crest. Cellular heterogeneity is a characteristic feature of both tumors and derived cell lines. Recent studies have revealed that both cell lines and tumors contain cancer stem cells. In culture, these cells are self-renewing, multipotent, and highly malignant; in tumors their frequency correlates with a worse prognosis. Their identification and characterization should now permit a targeted approach to more effective treatment of this often fatal childhood cancer.
