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Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

TIGAR, a p53-inducible regulator of glycolysis and apoptosis

Karim Bensaad1, Atsushi Tsuruta, Mary A Selak

  • 1The Beatson Institute for Cancer Research, Switchback Road, Glasgow G61 1BD, UK.

Cell
|July 15, 2006
PubMed

Insights

The tumor suppressor p53 regulates cell death via the TIGAR gene. TIGAR reduces reactive oxygen species (ROS) and glycolysis, protecting cells from p53-induced apoptosis and genomic damage.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cellular Metabolism

Background:

  • The p53 tumor-suppressor protein is crucial for preventing cancer.
  • p53 functions through cell-cycle arrest, apoptosis, and maintaining genome stability.

Purpose of the Study:

  • Identify novel p53-inducible genes.
  • Investigate the role of TIGAR (TP53-induced glycolysis and apoptosis regulator) in p53-mediated cellular responses.

Main Methods:

  • Analysis of p53-inducible gene expression.
  • Measurement of fructose-2,6-bisphosphate and intracellular reactive oxygen species (ROS) levels.
  • Assessment of cellular apoptosis and sensitivity to p53-induced death.

Main Results:

  • Identified TIGAR as a p53-inducible gene.
  • TIGAR expression decreased fructose-2,6-bisphosphate, inhibiting glycolysis and lowering intracellular ROS.
  • TIGAR protected cells from ROS-associated apoptosis; TIGAR knockdown sensitized cells to p53-induced death.

Conclusions:

  • TIGAR modulates the apoptotic response to p53, promoting cell survival under stress.
  • Reduced intracellular ROS by TIGAR contributes to p53's role in preventing genomic damage.

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