Related Experiment Videos
Surface-activated chemical ionization and high-flow gradient chromatography to reduce matrix effect
Simone Cristoni1, Luigi Rossi Bernardi, Piermario Gerthoux
1University of Milan, Centre for Bio-molecular Interdisciplinary Studies and Industrial Applications CISI, Via Fratelli Cervi 93, Segrate Milano, Italy. simone.cristoni@virgilio.it
Rapid Communications in Mass Spectrometry : RCM
|July 15, 2006
Summary
A new Surface-Activated Chemical Ionization (SACI) source works with high-flow chromatography to minimize matrix effects in drug analysis. This method improves quantitation accuracy for compounds like tacrolimus in biological samples.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Chromatography
Background:
- Matrix effects in liquid chromatography-mass spectrometry (LC-MS) analysis can cause significant quantitation errors.
- Classical electrospray ionization (ESI) sources are typically limited to low eluent flow rates, incompatible with high-flow gradient chromatography.
- High-flow gradient chromatography is desirable for reducing co-elution of analytes and matrix compounds, thereby minimizing matrix effects.
Purpose of the Study:
- To introduce and evaluate a novel atmospheric pressure chemical ionization source, Surface-Activated Chemical Ionization (SACI).
- To demonstrate the compatibility of SACI with high-flow gradient chromatography.
- To assess the reduction of matrix effects using SACI coupled with high-flow gradient chromatography.
Main Methods:
- Development and application of the Surface-Activated Chemical Ionization (SACI) source.
- Utilizing high-flow gradient chromatography (100-2000 microL/min).
- Analysis of tacrolimus in protein-precipitated blood samples.
Main Results:
- SACI operates effectively at high eluent flow rates, unlike traditional ESI sources.
- The combination of SACI and high-flow gradient chromatography significantly reduces matrix effects.
- Demonstrated reduction in quantitation errors for tacrolimus analysis in blood samples.
Conclusions:
- SACI is a viable ionization technique for high-flow gradient chromatography.
- This approach effectively mitigates matrix effects, improving analytical accuracy.
- The method shows promise for the analysis of immunosuppressive agents and other compounds in complex biological matrices.