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[Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse]
Yuan-ying Song1, Zhong Wang, Xun Qu
1The People's Hospital of Changchun.
Objective:
To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.
Methods:
Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.
Results:
The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.
Conclusion:
Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.
