Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Defined megadalton hyaluronan polymer standards.

Wei Jing1, F Michael Haller, Andrew Almond

  • 1Hyalose LLC, Oklahoma City, OK 73104, USA.

Analytical Biochemistry
|July 18, 2006
PubMed
Summary

Researchers created uniform synthetic hyaluronan (HA) complexes to serve as reliable polymer standards. These new HA standards offer improved calibration for estimating molecular mass, overcoming limitations of polydisperse materials.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The total cholesterol to high-density lipoprotein cholesterol ratio predicts post-stroke depression: linear association and individualized risk nomogram.

Lipids in health and disease·2026
Same author

-30°C-operable bioadhesive hydrogel sensors for embryonic-like skin regeneration and real-time wound monitoring.

Bioactive materials·2026
Same author

Near-point-of-care diagnosis of tuberculosis with LyocartE MTB Assay: a multicentre diagnostic accuracy study.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

CTLA-4 and CD154 expression on peripheral T cells as diagnostic biomarkers for pulmonary tuberculosis.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Integrated genomics and transcriptomics reveal key events in pancreatic neuroendocrine neoplasm.

BMC biology·2026
Same author

Lipid Nanoparticle Surface Engineering with Heparosan Polysaccharides for Safe and Effective mRNA Delivery <i>In Vitro</i> and <i>In Vivo</i>.

ACS applied materials & interfaces·2026

Area of Science:

  • Biochemistry
  • Polymer Science
  • Materials Science

Background:

  • Polydispersity in polymer standards limits accurate molecular mass estimation.
  • Hyaluronan (HA) is a crucial biopolymer with applications in medicine and research.
  • Developing well-defined HA standards is essential for precise characterization.

Purpose of the Study:

  • To synthesize monodisperse hyaluronan (HA) complexes for use as polymer standards.
  • To create HA standards with controlled molecular masses ranging from 1 to 8 megadaltons (MDa).
  • To establish reliable calibration tools for high-molecular weight HA analysis.

Main Methods:

  • Synthesized linear, narrow-distribution biotinylated HA chains via controlled in vitro reactions.
  • Complexed HA chains with streptavidin protein at substoichiometric levels.

Related Experiment Videos

  • Created complexes with varying numbers of HA chains (one to four) per streptavidin molecule.

    • Main Results:

    • Successfully prepared monodisperse synthetic hyaluronan (HA) complexes.
    • Generated HA complexes in the 1–8 megadalton (MDa) range.
    • Dendritic-like HA-streptavidin molecules exhibited mobility similar to natural HA on agarose gels.

    Conclusions:

    • The synthesized HA-streptavidin complexes serve as effective, defined size standards.
    • These standards address the limitations posed by polydispersity in traditional polymer calibration.
    • The new standards facilitate accurate molecular mass estimation for high-molecular weight hyaluronan preparations.