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Related Experiment Videos

Linking the human cytogenetic map with nucleotide sequence: the CCAP clone set.

Wonhee Jang1, Raluca Yonescu, Turid Knutsen

  • 1National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.

Cancer Genetics and Cytogenetics
|July 18, 2006
PubMed
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The Cancer Chromosome Aberration Project (CCAP) created a dataset linking human cytogenetic markers to genome sequences. This resource aids in studying genetic disorders and understanding genome sequence quality.

Area of Science:

  • Genomics
  • Cytogenetics
  • Cancer Research

Background:

  • Cytogenetic aberrations are hallmarks of many cancers and developmental disorders.
  • Accurate mapping of these aberrations to the human genome sequence is crucial for understanding their impact.
  • Existing resources may lack seamless integration between cytogenetic markers and nucleotide sequences.

Purpose of the Study:

  • To develop and provide a comprehensive dataset and clone repository (CCAP) for linking human cytogenetic markers with the human genome sequence.
  • To create a resource that facilitates the study of gene and sequence involvement in oncogenic and developmental disorders.
  • To assess the quality and coherence of the human genome sequence and its relationship to chromosomal banding patterns.

Main Methods:

Related Experiment Videos

  • Localization of 1,339 bacterial artificial chromosome (BAC) clones to chromosomal bands using high-resolution fluorescence in situ hybridization (FISH) mapping.
  • Positioning of BAC clones onto the primary human genome sequence at 1-2 Mb intervals.
  • Data accessibility through public Web sites and clone availability from a commercial repository.

    • Main Results:

    • 1,339 BAC clones were mapped to chromosomal bands.
    • 99.8% of BAC clones were positioned on the primary human genome sequence.
    • 95% of BAC clones were precisely located at or near their nucleotide start and stop points.

    Conclusions:

    • The CCAP BAC clone set provides essential anchors for investigating the role of chromosomal aberrations in disease.
    • The dataset offers a valuable tool for interrogating gene and sequence involvement in cancer and developmental disorders.
    • This resource enhances our understanding of genome sequence quality and the structural basis of chromosomal banding patterns.