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Published on: March 11, 2014
Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata
Fang Liu1, Jia-Bi Wang, Yue-Hua Liu
1Department of Dermatology, Beijing Chaoyang Hospital, Beijing 100020. roseliufang@sohu.com
Objective:
To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).
Methods:
HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).
Results:
The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.
Conclusion:
The sensibility of PCR is superior to that of immunofluorescence.
