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Updated: Aug 7, 2026

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Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
Published on: October 26, 2011
Derivation and characterization of hepatocytes from embryonic stem cells in vitro
Shigeaki Ishizaka1, Yukiteru Ouji, Masahide Yoshikawa
1Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|July 19, 2006
Abstract:
From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticipated. We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term.

