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Updated: Aug 7, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Metal-protein interactions: structure information from Ni(2+)-induced pseudocontact shifts in a native
Malene Ringkjøbing Jensen1, Jens J Led
1Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark.
Abstract:
Information about the structure of a native nonmetalloprotein was obtained from the pseudocontact shifts induced by a paramagnetic metal ion bound to the protein. The approach exploits the presence of metal binding sites on the surface of the protein. Using Escherichia coli thioredoxin as a model protein, we show that potential binding sites can be identified using the Cu(2+) ion, and that pseudocontact shifts induced by a Ni(2+) ion bound to one of these sites can provide valuable long-range structure information about the protein.
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