Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Statistical parametric mapping of immunopositive cell density.

Makoto Wada1, Kenji Yoshimi, Noriyuki Higo

  • 1Department of Physiology, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo, Tokyo, Japan. m-wada@med.juntendo.ac.jp

Neuroscience Research
|July 19, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heart Disease Mortality in Cancer Survivors: A Population-Based Study in Japan.

Journal of the American Heart Association·2023
Same author

RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.

Science advances·2023
Same author

Analysis of gut microbiome, host genetics, and plasma metabolites reveals gut microbiome-host interactions in the Japanese population.

Cell reports·2023
Same author

Comparison of surgical outcomes between iStent inject W implantation and microhook ab interno trabeculotomy in combination with phacoemulsification in primary open-angle glaucoma patients.

Frontiers in medicine·2023
Same author

Correction: Continuous Subcutaneous Foslevodopa/Foscarbidopa in Parkinson's Disease: Safety and Efficacy Results From a 12-Month, Single-Arm, Open-Label, Phase 3 Study.

Neurology and therapy·2023
Same author

Extracellular high molecular weight α-synuclein oligomers induce cell death by disrupting the plasma membrane.

NPJ Parkinson's disease·2023

This study introduces a novel method for analyzing cell densities in brain tissue, revealing significant microglial increases and dopamine neuron decreases after MPP+ injection in rats.

Area of Science:

  • Neuroscience
  • Immunohistochemistry
  • Computational Biology

Background:

  • Comparing cell densities across animal groups is crucial for understanding neuroinflammation and neurodegeneration.
  • Existing methods may lack the spatial resolution to detect localized changes in cell populations.

Purpose of the Study:

  • To develop and validate a new computational method for quantifying and mapping immunopositive cell densities.
  • To statistically compare cell densities between experimental groups on standardized brain sections.

Main Methods:

  • Developed a novel image analysis technique to automatically count immunopositive cells.
  • Created statistical parametric maps comparing cell densities pixel-by-pixel on normalized brain sections.
  • Applied the method to analyze Iba-1 (microglial) and tyrosine hydroxylase (dopaminergic neuron) staining in a rat model of Parkinson's disease.

Related Experiment Videos

Main Results:

  • Detected significant increases in microglial (Iba-1) density in response to MPP+ injection, extending beyond the substantia nigra.
  • Identified significant decreases in dopaminergic neuron (tyrosine hydroxylase) density in the MPP+-injected hemisphere.
  • The method successfully mapped and quantified changes in cell densities across entire immunostained sections.

Conclusions:

  • The developed method provides a robust and sensitive approach for analyzing spatial changes in cell densities in neurobiological research.
  • This technique is valuable for detecting localized neuroinflammation and neurodegeneration, aiding in the study of neurological disorders.
  • The findings highlight the utility of advanced imaging and statistical analysis in neuroscientific investigations.