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Related Experiment Videos

HTS technologies in biopharmaceutical discovery.

Ge Wu1, Stephen K Doberstein

  • 1Five Prime Therapeutics, 1650 Owens St., Suite 200, San Francisco, CA 94158, USA. ge.wu@FivePrime.com

Drug Discovery Today
|July 19, 2006
PubMed
Summary

High-throughput screening (HTS) principles enhance biopharmaceutical discovery by enabling systematic enumeration, cloning, production, and screening of secreted proteins. Optimizing key components is crucial for success in protein-based drug development.

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Area of Science:

  • Biopharmaceutical Discovery
  • Proteomics
  • Drug Development

Background:

  • Leveraging two decades of advancements in High-Throughput Screening (HTS), genomics, and parallel protein expression.
  • Enabling comprehensive and systematic enumeration, cloning, production, and screening of all secreted proteins.
  • Highlighting the integration of established HTS methodologies with novel protein-centric approaches.

Purpose of the Study:

  • To review the application of High-Throughput Screening (HTS) concepts to biopharmaceutical discovery.
  • To detail the operational components necessary for successful secreted protein discovery.
  • To compare and contrast small-molecule screening with protein screening methodologies.

Main Methods:

  • Systematic enumeration and cloning of secreted proteins.

Related Experiment Videos

  • High-throughput protein production and parallel expression.
  • Development and implementation of medically relevant assays and state-of-the-art screening.
  • Optimization of cDNA library construction, protein-sequence classification, and data management.

    • Main Results:

    • Demonstration of the feasibility of comprehensively screening secreted proteins.
    • Identification of critical operational components for successful biopharmaceutical discovery.
    • Illumination of common principles and key differences between small-molecule and protein screening.

    Conclusions:

    • High-throughput screening (HTS) strategies are highly applicable to discovering novel biopharmaceuticals.
    • Optimizing each stage of the discovery pipeline, from library construction to data management, is essential.
    • Understanding the parallels and distinctions between small-molecule and protein screening informs future drug discovery efforts.