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Related Experiment Videos

Three-dimensional adipose tissue model using low shear bioreactors.

Cynthia A Frye1, Charles W Patrick

  • 1Laboratory of Reparative Biology and Bioengineering, Department of Plastic Surgery, University of Texas M. D. Anderson Cancer Center, Houston, TX 77230-1402, USA.

In Vitro Cellular & Developmental Biology. Animal
|July 20, 2006
PubMed
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Researchers developed methods to grow large, viable three-dimensional (3-D) adipose-like tissues in bioreactors. These engineered tissues show potential for soft tissue engineering and reconstructive surgery applications.

Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Current limitations exist in culturing preadipocytes (PAs) for clinically relevant adipose tissue volumes.
  • Soft tissue engineering strategies in reconstructive surgery require larger volumes of functional adipose tissue.

Purpose of the Study:

  • To develop techniques for culturing and analyzing three-dimensional (3-D) adipose-like tissues.
  • To overcome limitations in current preadipocyte culture methods.
  • To generate clinically relevant volumes of adipose tissue for reconstructive surgery.

Main Methods:

  • Culture of 3T3-L1 cells in a rotating-wall bioreactor, with and without microcarriers.
  • Visualization and analysis techniques for 3-D PA-like tissues.

Related Experiment Videos

  • Histology and in vivo animal studies for aggregate analysis.
  • Use of a perfused rotating-wall bioreactor to generate tissue aggregates.

    • Main Results:

    • Achieved viable PA-like tissues up to 4-5 mm in diameter without microcarriers.
    • Demonstrated lipid-loading capacity in the engineered tissues.
    • Successfully generated large tissue aggregates in bioreactor culture systems.

    Conclusions:

    • Developed successful strategies for culturing large, viable 3-D adipose-like tissues.
    • The engineered tissues hold promise for soft tissue engineering applications.
    • Bioreactor culture systems can effectively generate clinically relevant adipose tissue volumes.