Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reflection confocal microscopy for quantitative assessment of airway surface layer dysregulation and pharmacological rescue in cystic fibrosis under near-physiological conditions.

Scientific reports·2025

Same author

Nitrogen concentration shapes the size structure and the functional diversity of phytoplankton communities in the southern Indian Ocean.

ISME communications·2025

Same author

Origins of chromosome instability unveiled by coupled imaging and genomics.

Nature·2025

Same author

Hydrophobic interactions of FG-nucleoporins are required for dilating nuclear membrane pores into selective transport channels after mitosis.

bioRxiv : the preprint server for biology·2025

Same author

Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.

Molecular systems biology·2025

Same author

Enabling global image data sharing in the life sciences.

Nature methods·2025

Same journal

Human aminoacyl-tRNA synthetases as integrators of translation and cell signalling networks.

Nature reviews. Molecular cell biology·2026

Same journal

How proteins fold.

Nature reviews. Molecular cell biology·2026

Same journal

Single-cell evidence for PANoptosome complexes.

Nature reviews. Molecular cell biology·2026

Same journal

Reply to 'Single-cell evidence for PANoptosome complexes'.

Nature reviews. Molecular cell biology·2026

Same journal

Plucking cellular ribosomes with Ribo-Tweezer.

Nature reviews. Molecular cell biology·2026

Same journal

COPII meets autophagy at the ER membrane.

Nature reviews. Molecular cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 2, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

High-throughput fluorescence microscopy for systems biology.

Rainer Pepperkok¹, Jan Ellenberg

¹Cell Biology/Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. rainer.pepperkok@embl.de

Nature Reviews. Molecular Cell Biology

|July 20, 2006

Summary

This summary is machine-generated.

Defining gene function requires advanced imaging. Fluorescence microscopy is becoming a quantitative, high-throughput tool for functional genomics in cells.

More Related Videos

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Related Experiment Videos

Last Updated: May 2, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Area of Science:

Genomics
Cell Biology
Biotechnology

Background:

The post-genomic era necessitates genome-wide gene function definition.
Cells are the fundamental units of biological processes.
Fluorescence microscopy is a key technique for studying cellular processes in vivo.

Purpose of the Study:

To highlight the transition of fluorescence microscopy towards quantitative and high-throughput applications.
To emphasize the importance of fluorescence microscopy in functional genomics.

Main Methods:

Quantitative fluorescence microscopy.
High-throughput imaging techniques.
Functional genomics assays in living cells.

Main Results:

Fluorescence microscopy is evolving into a quantitative tool.
The technology is becoming suitable for high-throughput functional genomics.

Conclusions:

Quantitative, high-throughput fluorescence microscopy is essential for advancing functional genomics.
This technological shift enables comprehensive gene function analysis in model organisms and humans.