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[Preliminary application on test paper of gold-labelled antibody against fibronectin EIIIA splicing variant]
Ning-Guo Liu1, Yi-Jiu Chen, Ren-Quan Lu
1Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China. liuningguo@yahoo.com.cn
Objective:
To develop the test Paper of gold-labelled antibody against fibronectin EIIIR A splicing variant, which can be available in forensic wound interval estimation.
Methods:
Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats.
Results:
On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining.
Conclusion:
The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.
