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Microbial contamination of antiseptics and disinfectants
Somwang Danchaivijitr1, Chertsak Dhiraputra, Yong Rongrungruang
1Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bankok. sisdc@mahidol.ac.th
Objective:
To study the bacterial contamination of antiseptics and disinfections in-use and the risk factors for contamination.
Material And Method:
Bacterial contamination of antiseptics and disinfectants was done by culturing in-use solutions. Eight commonly used solutions were studied: alcohol 70%, chlorhexidine 4%, and 0.5%, povidone iodine 7.5% and 10%, tincture iodine 1-2%, lysol 2% and sodium hypochlorite 0.5%.
Results:
The following risk factors for contamination were found : preparation by unskilled personnel, improper containers and prolonged use. Contamination with bacteria were found in 1.8% of 16,142 samples tested Highest rate of contamination was found in Lysol 2%. There was no contamination of povidone iodine 10% and tincture iodine 1-2%. Bacterial contamination of antiseptics and disinfectants was highest in provincial hospitals and was not found in university hospitals. The rates of contamination correlated with the duration of use. Most bacteria isolated were those found in the environment.
Conclusion:
The contamination of in-use antiseptics and disinfectants was as high as 1.8%. Risk factors for contamination were improper preparation and prolonged use.
Insights
Bacterial contamination of in-use antiseptics and disinfectants reached 1.8%. Key risk factors included improper preparation and prolonged use, with Lysol 2% showing the highest contamination rates.
Area of Science:
- Microbiology
- Infection Control
- Hospital Hygiene
Background:
- Antiseptics and disinfectants are crucial for infection control.
- Contamination of these solutions can pose a significant risk.
- Understanding contamination sources is vital for patient safety.
Purpose of the Study:
- To investigate the prevalence of bacterial contamination in commonly used antiseptics and disinfectants.
- To identify risk factors associated with the contamination of these solutions.
- To compare contamination rates across different types of healthcare facilities.
Main Methods:
- Culturing of in-use solutions of eight common antiseptics and disinfectants.
- Analysis of 16,142 samples from various healthcare settings.
- Identification of risk factors including preparation methods, containers, and duration of use.
Main Results:
- A 1.8% contamination rate was observed across all tested solutions.
- Lysol 2% exhibited the highest contamination rate; povidone iodine 10% and tincture iodine 1-2% showed no contamination.
- Contamination was more prevalent in provincial hospitals compared to university hospitals and correlated with prolonged use.
Conclusions:
- In-use antiseptics and disinfectants can be significantly contaminated (up to 1.8%).
- Improper preparation and prolonged use are identified as primary risk factors.
- Environmental bacteria were the most common isolates, highlighting the importance of sterile handling practices.
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