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[Determination of artemisinin in Artemisia annuna by HPLC-ELSD]
Ao-Ao Zhou1, Wen-Xin Zheng, Fa-Huan Ge
1National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China.
Objective:
A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method.
Methods:
The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.6 mm, 5 microm) column. The temperature of detector was 40 degrees C, the pressure of N2 was 3.5 bar, and gain value was 9.
Results:
The calibration curves were linear within the range of 1 - 5 microg, and the average recovery was 99.33% (RSD = 1.97%).
Conclusion:
The method was rapid, convenient, and accurate. It can be used for the quality control of this herbal medicine.
