Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrostatic contributions to indole-lipid interactions.

Holly C Gaede1, Wai-Ming Yau, Klaus Gawrisch

  • 1Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Room 3N07, Bethesda, Maryland 20892-9410, USA.

The Journal of Physical Chemistry. B
|July 21, 2006
PubMed
Summary

Electrostatic forces influence indole

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Nanopore Confinement on the Conformational, Dynamical, and Self-Assembly Properties of an FG-Repeat Peptide.

The journal of physical chemistry. B·2025
Same author

Effects of Nanopore Confinement on the Conformational, Dynamical, and Self-Assembly Properties of an FG-Repeat Peptide.

bioRxiv : the preprint server for biology·2025
Same author

Small angle neutron scattering study of rhodopsin oligomerization and G-protein coupling in a physiologically relevant lipid membrane.

Biochimica et biophysica acta. Biomembranes·2025
Same author

Solid-state NMR of membrane peptides and proteins in the lipid cubic phase.

Biophysical journal·2025
Same author

Conformations of a low-complexity protein in homogeneous and phase-separated frozen solutions.

Biophysical journal·2024
Same author

Conformations of a Low-Complexity Protein in Homogeneous and Phase-Separated Frozen Solutions.

bioRxiv : the preprint server for biology·2024

Area of Science:

  • Biophysics
  • Membrane Biophysics

Background:

  • Indole interactions with lipids are crucial for membrane protein function.
  • Understanding these interactions informs membrane structure and dynamics.

Purpose of the Study:

  • To elucidate the role of electrostatic forces in indole-lipid interactions.
  • To determine indole's distribution and orientation within phospholipid bilayers.

Main Methods:

  • (1)H and (2)H Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Nuclear Overhauser effect (NOE) spectroscopy.
  • Utilized various phospholipid bilayers with incorporated indole.

Main Results:

  • Indole exhibits a bimodal distribution, localizing near the glycerol region and the choline headgroup.
  • Two-thirds of indole prefers the upper hydrocarbon/glycerol region with a specific orientation.
  • Cation-pi interactions with choline drive indole's association with the headgroup.

Conclusions:

  • Electrostatic forces, particularly cation-pi interactions, significantly influence indole's membrane localization.
  • Indole-lipid association lifetime is approximately 30 ns.
  • Findings impact understanding of tryptophan-rich membrane proteins and membrane structural changes.

Related Experiment Videos