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Updated: Aug 7, 2026

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
Published on: December 4, 2016
Characterization of heterogeneously functionalized dendrimers by mass spectrometry and EPR spectroscopy
Eric D Walter1, Karl B Sebby, Robert J Usselman
1Department of Chemistry and Biochemistry, OpTeC, and Center for Bioinspired Nanomaterials, 108 Gaines Hall, Montana State University, Bozeman, Montana 59717, USA.
Abstract:
Starburst dendrimers are receiving considerable attention as templates for the assembly of structured arrays of molecular components. This research motivates the development of improved methods for dendrimer characterization-specifically, for determining the numbers, distributions of numbers, and spatial distribution of molecular species synthetically attached to macromolecular templates. Such information provides the basis for advancing strategies aimed at controlling dendrimer functionalization, and thus represents enabling technology for tailoring the composition and structure of molecular arrays fashioned on dendrimer templates. Moreover, this information is vital to the proper interpretation of ongoing experiments in which dendrimers sparsely functionalized with reporter groups are used as probes. In this article, we report MALDI-TOF mass spectrometry and EPR spectroscopy of heterogeneously functionalized G(4)-PAMAM dendrimers bearing nitroxide spin-labels.
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