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Updated: Aug 7, 2026
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
Published on: March 1, 2016
Functionalization of solid surfaces with thermoresponsive protein-resistant films
D O H Teare1, D C Barwick, W C E Schofield
1Department of Chemistry, Science Laboratories, Durham University, Durham DH1 3LE, England, UK.
Thermoresponsive poly(N-isopropylacrylamide) films were created using pulsed plasma polymerization. These surfaces exhibit reversible protein adsorption, switching behavior with temperature changes, demonstrated by SPR and fluorescence microscopy.
Area of Science:
- Materials Science
- Polymer Chemistry
- Surface Science
Background:
- Thermoresponsive polymers change properties with temperature.
- Poly(N-isopropylacrylamide) (PNIPAM) is a well-known thermoresponsive polymer.
- Plasma polymerization offers precise control over film deposition.
Purpose of the Study:
- To deposit thermoresponsive PNIPAM films using pulsed plasma polymerization.
- To investigate the reversible protein adsorption behavior of these PNIPAM surfaces.
- To characterize the temperature-dependent switching of surface properties.
Main Methods:
- Pulsed plasma polymerization of N-isopropylacrylamide.
- Surface Plasmon Resonance (SPR) for protein adsorption analysis.
- Fluorescence microscopy for visualizing protein binding.
Main Results:
- Successfully deposited thermoresponsive PNIPAM films.
- Demonstrated reversible adsorption of fibrinogen and bovine serum albumin (BSA).
- Confirmed temperature-dependent switching of protein adsorption at low and elevated temperatures.
Conclusions:
- Pulsed plasma polymerization is effective for creating thermoresponsive PNIPAM films.
- These films exhibit tunable surface properties for controlled protein interactions.
- The demonstrated reversible behavior has potential applications in biosensing and biomaterials.
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