Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oncogenes and tumor-suppressor genes.

T A Lehman1, R Reddel, A M Peiifer

  • 1Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.

Environmental Health Perspectives
|June 1, 1991
PubMed
Summary

Oncogenes like v-Ha-ras and Ki-ras can cause lung cancer when introduced into cells. Tumor suppressor genes, such as p53, are crucial for preventing lung carcinoma development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversible lysine-specific demethylase 1 antagonist HCI-2509 inhibits growth and decreases c-MYC in castration- and docetaxel-resistant prostate cancer cells.

Prostate cancer and prostatic diseases·2016
Same author

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis.

Osteoarthritis and cartilage·2016
Same author

Consent down under.

British dental journal·2015
Same author

Immunocytochemical analysis of oncoproteins and growth-factors in human-malignant mesothelioma.

Oncology reports·2011
Same author

Hypoglossal nerve palsy, trigeminal nerve palsy and Horner's syndrome in association with epidural block.

International journal of obstetric anesthesia·2009
Same author

The effects of esomeprazole combined with aspirin or rofecoxib on prostaglandin E2 production in patients with Barrett's oesophagus.

Alimentary pharmacology & therapeutics·2006

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oncogenes play a role in human lung carcinogenesis.
  • Identifying tumor-suppressor genes is crucial for understanding and treating lung carcinomas.

Purpose of the Study:

  • Investigate the functional role of oncogenes in lung cancer development.
  • Define negative growth-regulating genes with tumor-suppressive effects in human lung carcinomas.

Main Methods:

  • Transfection of activated oncogenes (v-Ha-ras, Ki-ras, myc, raf) into bronchial epithelial cells (BEAS-2B).
  • Loss of heterozygosity studies on chromosome 17p.
  • Monochromosome-cell and cell-cell fusion studies.
  • Investigating the role of p53 and Rb-1 tumor-suppressor genes.

Related Experiment Videos

Main Results:

  • Transfection of v-Ha-ras, Ki-ras, or myc/raf combination induced tumorigenicity in BEAS-2B cells.
  • Transfection of raf or myc alone did not produce tumorigenic cell lines.
  • Consistent allelic DNA deletions on chromosome 17p were observed in squamous cell carcinomas.
  • Mutations in the p53 tumor-suppressor gene were detected.
  • Hybrid cell clones from fusions were generally nontumorigenic.

Conclusions:

  • Specific oncogenes can induce tumorigenicity in bronchial epithelial cells.
  • Chromosome 17p alterations and p53 mutations are implicated in squamous cell carcinoma development.
  • Tumor-suppressor genes likely play a critical role in inhibiting lung carcinogenesis.