Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allelotyping of human prostatic adenocarcinoma.

K Kunimi1, U S Bergerheim, I L Larsson

  • 1Ludwig Institute for Cancer Research, Stockholm, Sweden.

Genomics
|November 1, 1991
PubMed
Summary

Allelotyping revealed genetic information loss in 61% of prostate adenocarcinoma cases. Deletions on chromosomes 16, 8, and 10 were frequent, correlating with advanced disease and higher malignancy grade.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunogenicity of subcutaneous TNF inhibitors and its clinical significance in real-life setting in patients with spondyloarthritis.

Rheumatology international·2021
Same author

Minor effect of loss to follow-up on outcome interpretation in the Swedish spine register.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2019
Same author

Long-term outcome after spinal fusion for isthmic spondylolisthesis in adults.

The spine journal : official journal of the North American Spine Society·2018
Same author

Three out of four disease-modifying anti-rheumatic drug-naïve rheumatoid arthritis patients meet 28-joint Disease Activity Score remission at 12 months: results from the FIN-ERA cohort.

Scandinavian journal of rheumatology·2017
Same author

Arterial stiffness is increased in young women with endometriosis.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·2014
Same author

Difference in the ratio of high-molecular weight (HMW) to total adiponectin and HMW adiponectin in late post-menopausal women.

Journal of endocrinological investigation·2013

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Prostate adenocarcinoma is a significant health concern.
  • Understanding genetic alterations in prostate cancer is crucial for diagnosis and treatment.
  • Allelic deletions are common genetic events in cancer development.

Purpose of the Study:

  • To investigate the frequency and patterns of allelic deletions in prostate adenocarcinoma.
  • To identify specific chromosomal regions frequently affected by genetic loss.
  • To correlate allelic deletion status with tumor malignancy and disease stage.

Main Methods:

  • Allelotyping was performed on 18 prostate adenocarcinoma specimens.
  • Restriction fragment length polymorphism (RFLP) probes were used to detect allelic deletions.

Related Experiment Videos

  • Analysis focused on specific chromosomal arms, including 16q, 8p, 10p, 10q, and 18q.

    • Main Results:

    • Allelic deletions were observed in 61% (11 of 18) of prostate adenocarcinoma specimens.
    • Frequent deletions occurred on chromosome 16q (60%), 8p (50%), and chromosome 10 (10p: 55%, 10q: 30%).
    • Deletions on chromosomes 8, 10, or 16 were necessary for other allelic losses; advanced tumors showed deletions.

    Conclusions:

    • Allelic deletions are prevalent in prostate adenocarcinoma, particularly on chromosomes 8, 10, and 16.
    • The presence of multiple allelic deletions correlates with higher tumor malignancy grade.
    • Allelic deletion patterns may serve as biomarkers for advanced prostate cancer and disease progression.