Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacterial quorum sensing and cell surface electrokinetic properties.

K E Eboigbodin1, J R A Newton, A F Routh

  • 1Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, UK.

Applied Microbiology and Biotechnology
|July 21, 2006
PubMed
Summary

Quorum sensing significantly impacts bacterial surface charge and electrokinetic properties. This study reveals differences in Escherichia coli strains, highlighting quorum sensing

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrosion inhibitor distribution on abrasive-blasted steels.

Journal of colloid and interface science·2022
Same author

Auto-stratification in drying colloidal dispersions: a diffusive model.

Journal of colloid and interface science·2012
Same author

Bacterial water quality and network hydraulic characteristics: a field study of a small, looped water distribution system using culture-independent molecular methods.

Journal of applied microbiology·2012
Same author

Autostratification in drying colloidal dispersions: experimental investigations.

Langmuir : the ACS journal of surfaces and colloids·2012
Same author

Shear rheology of molten crumb chocolate.

Journal of food science·2009
Same author

Effect of water on overbased sulfonate engine oil additives.

Langmuir : the ACS journal of surfaces and colloids·2008

Area of Science:

  • Microbiology
  • Biophysics
  • Surface Chemistry

Background:

  • Quorum sensing (QS) is a cell-to-cell communication mechanism in bacteria.
  • Bacterial surface electrokinetic properties influence interactions with environments and other cells.
  • The role of QS in modulating bacterial surface properties remains underexplored.

Purpose of the Study:

  • To investigate the influence of quorum sensing on the electrokinetic properties of Escherichia coli.
  • To compare the surface charge and electrophoretic mobility of wild-type and quorum sensing-deficient E. coli strains.

Main Methods:

  • Utilized Escherichia coli MG1655 wild-type and a LuxS- mutant (deficient in AI-2 production).
  • Quantified Autoinducer-2 (AI-2) using the Vibrio harveyi bioassay.

Related Experiment Videos

  • Measured electrophoretic mobility via phase amplitude light-scattering (PALS) to determine Zeta potential.

    • Main Results:

    • AI-2 production levels correlated with bacterial growth phases and glucose presence.
    • Significant differences in electrophoretic mobility were observed between the wild-type and LuxS- mutant strains.
    • Bacterial surface charge was dependent on growth phase and quorum sensing capability.

    Conclusions:

    • Quorum sensing plays a significant role in determining bacterial surface chemistry.
    • The ability to participate in quorum sensing influences the electrokinetic properties of Escherichia coli.
    • These findings provide insights into QS-mediated regulation of bacterial surface characteristics.