Related Experiment Video
Updated: Aug 7, 2026
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
Published on: March 24, 2020
[Relationship between the injury time and DNA fragmentation and its quantity in rat brain]
Fan Li1, Zhi-Gang Liao, Shu-Ling Ma
1West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. fanlfy@mail.haust.edu.cn
Objective:
To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.
Methods:
The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.
Results:
With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.
Conclusion:
TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.
